Anti-inflammatory effect of alpha lipoic acid loaded calcium citrate nanoparticle on human keratinocyte HaCaT cells

Authors

  • Boonyaras Bukkavesa Police General Hospital, Bangkok, Thailand
  • Siwaporn Nilyai Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Kanidta Sooklert Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Sasin Thamakaison Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Amornpun Sereemaspun

Keywords:

Alpha lipoic acid, calcium citrate nanoparticle, anti-inflammation

Abstract

Background: Alpha lipoic acid (LA) is potent anti-oxidant and anti-inflammation natural compound. To enhance the efficacy and stability of LA, LA-loaded calcium citrate nanoparticles were developed.

Objective: To investigate the anti-inflammatory effects of LA-loaded calcium citrate nanoparticles (LA-NPs) in HaCaT keratinocytes.

Methods: HaCaT cells were exposed under two inflammation stimuli conditions, lipopolysaccharide (LPS) and Pb(NO3 ) 2 , and then were treated with or without LA and LA-NPs for 24 h. Cell viability and pattern of cell death were evaluated. In addition, the expression levels of inflammatory cytokines including IL-1gif.latex?\beta, IL-6, and TNF-gif.latex?\alpha as well as inflammatory mediator COX-2 were determined by real-time polymerase chain reaction.

Results: LA and LA-NPs decreased the percentage of cell death in both LPS and Pb(NO3 ) 2 -induced conditions. In LPS-induced cells, LA and LA-NPs attenuated the fold of gene expression levels in IL-1gif.latex?\beta, IL-6, TNF-gif.latex?\alpha, and COX-2. Decreased expression in proinflammatory cytokines, including IL-1gif.latex?\beta and IL-6, was also observed in Pb(NO3 ) 2 -induced cells.

Conclusion: Our study demonstrated the anti-inflammatory effects of LA and LA-NPs on LPS and Pb(NO3 ) 2 - induced human keratinocytes.

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Published

2023-10-17

How to Cite

1.
Bukkavesa B, Nilyai S, Sooklert K, Thamakaison S, Sereemaspun A. Anti-inflammatory effect of alpha lipoic acid loaded calcium citrate nanoparticle on human keratinocyte HaCaT cells. Chula Med J [Internet]. 2023 Oct. 17 [cited 2024 Oct. 12];65(1). Available from: https://he05.tci-thaijo.org/index.php/CMJ/article/view/1070