Anti-inflammatory effect of alpha lipoic acid loaded calcium citrate nanoparticle on human keratinocyte HaCaT cells

Boonyaras  Bukkavesa; Siwaporn  Nilyai; Kanidta  Sooklert; Sasin  Thamakaison; Amornpun  Sereemaspun

Authors

Boonyaras Bukkavesa Police General Hospital, Bangkok, Thailand
Siwaporn Nilyai Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
Kanidta Sooklert Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
Sasin Thamakaison Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
Amornpun Sereemaspun

Keywords:

Alpha lipoic acid, calcium citrate nanoparticle, anti-inflammation

Abstract

Background: Alpha lipoic acid (LA) is potent anti-oxidant and anti-inflammation natural compound. To enhance the efficacy and stability of LA, LA-loaded calcium citrate nanoparticles were developed.

Objective: To investigate the anti-inflammatory effects of LA-loaded calcium citrate nanoparticles (LA-NPs) in HaCaT keratinocytes.

Methods: HaCaT cells were exposed under two inflammation stimuli conditions, lipopolysaccharide (LPS) and Pb(NO₃ ) ₂ , and then were treated with or without LA and LA-NPs for 24 h. Cell viability and pattern of cell death were evaluated. In addition, the expression levels of inflammatory cytokines including IL-1 $gif.latex?\beta$ , IL-6, and TNF- $gif.latex?\alpha$ as well as inflammatory mediator COX-2 were determined by real-time polymerase chain reaction.

Results: LA and LA-NPs decreased the percentage of cell death in both LPS and Pb(NO₃ )₂ -induced conditions. In LPS-induced cells, LA and LA-NPs attenuated the fold of gene expression levels in IL-1 $gif.latex?\beta$ , IL-6, TNF- $gif.latex?\alpha$ , and COX-2. Decreased expression in proinflammatory cytokines, including IL-1 $gif.latex?\beta$ and IL-6, was also observed in Pb(NO₃ ) ₂ -induced cells.

Conclusion: Our study demonstrated the anti-inflammatory effects of LA and LA-NPs on LPS and Pb(NO₃ ) ₂ - induced human keratinocytes.

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