Prevalence and distribution of HPV type in King Chulalongkorn Memorial Hospital

Abstract

Objective : To investigate the prevalence and distribution of human papillomavirus (HPV) type in specimens collecting at King Chulalongkorn Memorial Hospital

Design : Cross-sectional descriptive study

Setting : Virology Laboratory Unit, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand

Subjects : All 139 clinical samples were sent to Virology Laboratory Unit, from October 2006 to May 2007

Methods : All 139 samples were primarily screen positive for the presence of HPV-DNA by Amplicore HPV amplification kit (Roche, USA). They were then continued for typing using Linear Array HPV genotyping kit purchased from Roche.

Results : Only 123 samples were from women. Sixty-one samples (49.59%) were single infection with 47 (77.05 %) were high-risk type and 14 (22.95 %) were lowrisk type. Another 62 (50.41 °/o) samples were mixed infection of two types (33; 53.23 %)1 three types (19; 30.64 7o) and equal or grater than four types (10; 16.13 %). Among these types, HPV -52 which belongs to high- risk types were of the highest prevalence (31;25.20 %) followed by type 16 (30; 24.39 %)

Conclusion : Although the data obtained from laboratory investigation might not be a good representative the figure of the prevalence of the population, at least it is useful for estimating the distribution ofHPV type of Thai women. Our results indicated that most Thai women were infected with high-risk types of HPV especially HPV-52 and -16.