Four-week induction of type 2 diabetes mellitus in rats by streptozotocin and high-fat diet

Authors

  • Wirin Bowonsomsarit Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Weerapat Anegkamol Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Natthaya Chuaypen Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Maroot Kaewwongse School of Medical Sciences, University of Phayao, Phayao, Thailand
  • Piyaratana Tosukhowong Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Phisit Prapunwattana Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Thasinas Dissayabutra Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

Keywords:

Type 2 diabetes, animal model, high-fat diet, streptozotocin

Abstract

Background: Current rodent models for diabetes mellitus type 2 could be divided into genetic-engineered and environmental-induced. Since genetic-modified rodents are difficult to acquire and costly for Thai researchers, we tried to develop a protocol for food-induced diabetic rats.

Objectives: To develop a convenient and economical type 2 diabetic mellitus rats with a short course (4 - week) of induction for research purpose.

Methods: Eight-week-old male Wistar rats were divided into two groups: control group and streptozotocin with high-fat diet (STZ-HFD) group (n = 4 each). The control group received normal chow (c.p.082 diet with 20.0% energy from fat). For the STZ-HFD group, STZ (30 mg/kg) was intraperitoneal injected at week 0 of experiment with HFD feeding (58.0% energy from fat). Rats were fed ad libitum. Blood samples were collected for diabetic evaluation weekly for 4 weeks.

Results: The body weight of the STZ-HFD group was significantly higher than that of the control group. Blood glucose, plasma insulin, homeostasis model assessment of insulin resistance (HOMA-IR) index and HbA1C were increased in the STZ-HFD group. Moreover, STZ-HFD rats suffered with a non-alcoholic fatty liver disease.

Conclusion: Low dose STZ injection (30 mg/kg) with HFD feeding (58.0% energy from fat) could be used to induced type 2 diabetes in rats within 4 weeks.

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Published

2023-07-20

How to Cite

1.
Bowonsomsarit W, Anegkamol W, Chuaypen N, Kaewwongse M, Tosukhowong P, Prapunwattana P, Dissayabutra T. Four-week induction of type 2 diabetes mellitus in rats by streptozotocin and high-fat diet. Chula Med J [Internet]. 2023 Jul. 20 [cited 2024 Nov. 6];65(4). Available from: https://he05.tci-thaijo.org/index.php/CMJ/article/view/167