Tea polyphenols extracted from fresh young tea leaves exhibits antibacterial activity against some bacterial species: An in vitro study

Belal  Hossain Sikder; Shafiul  Alam; Shakir  Moazzem; Hazrat  Ali; Abdullah  Al-Mamun; Shah  Samiur Rashid

Authors

Belal Hossain Sikder Shahjalal University of Science and Technology, Bangladesh
Shafiul Alam Shahjalal University of Science and Technology, Bangladesh
Shakir Moazzem Shahjalal University of Science and Technology, Bangladesh
Hazrat Ali Shahjalal University of Science and Technology, Bangladesh
Abdullah Al-Mamun Shahjalal University of Science and Technology, Bangladesh
Shah Samiur Rashid Universiti Malaysia Pahang, Kuantan, Malaysia

Keywords:

Antibacterial activity, polyphenols, tea leaves, inhibitive effects, bacterial isolates

Abstract

Background: Natural products are rich sources of therapeutic agents for remedy, prevention and cure of diseases. Currently, many strains of bacteria have become resistant to many of the conventional antibiotics. So, in-depth researches should be focused on the antibacterial activity of natural compounds derived from natural sources.

Objectives: The purposes of this study were to determine the antibacterial activity of polyphenols extracted from fresh young tea leaves against Escherichia coli, Pseudomonas spp., Klebsiella spp. and Staphylococcus aureus.

Methods: Antibacterial activity of young and fresh leaves of tea was evaluated by disc diffusion method and well diffusion method. The results were reported in zone diameter in millimeter as mean  standard deviation of triplicates (n = 3).

Results: We found that polyphenols extracted from fresh young tea leaves showed potential inhibitory activity against bacterial growth. The diameters of the inhibition zone found for extracted polyphenols from young fresh tea leaves showed satisfactory antibacterial activity against the selected bacterial isolates. In disc diffusion method, polyphenol extracts have shown effective results against Pseudomonas spp. (19.33 $gif.latex?\pm$ 2.52 at 100 mg/ml concentration) and Staphylococcus aureus (18.00 $gif.latex?\pm$ 3.61 at 75 mg/ml concentration). In well diffusion method, the polyphenol extracts have shown effective results against Pseudomonas spp. (17.00 $gif.latex?\pm$ 2.65 at 100 mg/ml) and E. coli (15.00 $gif.latex?\pm$ 2 at 100 mg/ml).

Conclusion: Polyphenols extracted from fresh young tea leaves have potential inhibitive effects against growth of the selected bacterial isolates and can be used as an antibacterial agent.

Downloads

Download data is not yet available.

References

Najafi-Taher R, Ghaemi B, Kharrazi S, Rasoulikoohi S, Amani A. Promising antibacterial effects of silver nanoparticle-loaded tea tree oil nanoemulsion: A synergistic combination against resistance threat. AAPS Pharm Sci Tech 2018;19:1133-40. https://doi.org/10.1208/s12249-017-0922-y

Shahriary M, Veisi H, Hekmati M, Hemmati S. In situ green synthesis of Ag nanoparticles on herbal tea extract (Stachys lavandulifolia)-modified magnetic iron oxide nanoparticles as antibacterial agent and their 4-nitrophenol catalytic reduction activity. Mater Sci Eng C Mater Biol Appl 2018;90:57-66.

https://doi.org/10.1016/j.msec.2018.04.044

Arun SD, Minal MK, Karibasappa GN, Prashanth VK, Girija AD, Harish CJ. Comparative assessment of antibacterial efficacy of aqueous extract of commercially available black, green, and lemon tea: an in vitro study. Int J Health Sci (Qassim) 2017;11:42-6.

Shetta A, Kegere J, Mamdouh W. Comparative study of encapsulated peppermint and green tea essential oils in chitosan nanoparticles: Encapsulation, thermal stability, in-vitro release, antioxidant and antibacterial activities. Int J Biol Macromol 2019;126:731-42.

https://doi.org/10.1016/j.ijbiomac.2018.12.161

Hafiz I, Bhatti HN, Hanif MA, Shahid M. In-vitro antibacterial and antioxidant potential of winged prickly ash, green tea and thyme. J Biol Regul Homeost Agents 2018;32:101-6.

Salvatori C, Barchi L, Guzzo F, Gargari M. A comparative study of antibacterial and anti-inflammatory effects of mouthrinse containing tea tree oil. Oral Implantol (Rome) 2017;10:59-70.

https://doi.org/10.11138/orl/2017.10.1.059

Muthu M, Gopal J, Min SX, Chun S. Green tea versus traditional Korean teas: antibacterial/ antifungal or both? Appl Biochem Biotechnol 2016;180:780-90.

https://doi.org/10.1007/s12010-016-2132-6

Zheng S, Hao L, Zhang L, Wang K, Zheng W, Wang X, et al. Tea polyphenols functionalized and reduced graphene oxide-ZnO composites for selective Pb(2+) removal and enhanced antibacterial activity. J Biomed Nanotechnol 2018;14:1263-76. https://doi.org/10.1166/jbn.2018.2584

Chen Z, Lv X, Zhao M, Zhang P, Ren X, Mei X. Encapsulation of green tea polyphenol by pH responsive, antibacterial, alginate microgels used for minimally invasive treatment of bone infection. Colloids Surf B Biointerfaces 2018;170:648-55. https://doi.org/10.1016/j.colsurfb.2018.06.065

Wittpahl G, Kolling-Speer I, Basche S, Herrmann E, Hannig M, Speer K, et al. The polyphenolic composition of cistus incanus herbal tea and its antibacterial and anti-adherent activity against Streptococcus mutans. Planta Med 2015;81:1727-35. https://doi.org/10.1055/s-0035-1557822

Kaur A, Gabrani R, Dang S. Nanoemulsions of green tea catechins and other natural compounds for the treatment of urinary tract infection: Antibacterial analysis. Adv Pharm Bull 2019;9:401-8.

https://doi.org/10.15171/apb.2019.047

Finotti E, Bersani E, Friedman M. Application of a functional mathematical index for antibacterial and anticarcinogenic effects of tea catechins. J Agric Food Chem 2011;59:864-9.

https://doi.org/10.1021/jf101717t

Parvez MAK, Saha K, Rahman J, Munmun RA, Rahman MA, Dey SK, et al. Antibacterial activities of green tea crude extracts and synergistic effects of epigallocatechingallate (EGCG) with gentamicin against MDR pathogens. Heliyon 2019;5:e02126. https://doi.org/10.1016/j.heliyon.2019.e02126

Park H, Jang CH, Cho YB, Choi CH. Antibacterial effect of tea-tree oil on methicillin-resistant Staphylococcus aureus biofilm formation of the tympanostomy tube: an in vitro study. In Vivo 2007;21:1027-30.

Cho YS, Schiller NL, Oh KH. Antibacterial effects of green tea polyphenols on clinical isolates of methicillinresistant Staphylococcus aureus. Curr Microbiol 2008;57:542-6.

https://doi.org/10.1007/s00284-008-9239-0

Toda M, Okubo S, Hara Y, Shimamura T. Antibacterial and bactericidal activities of tea extracts and catechins against methicillin resistant Staphylococcus aureus. Nihon Saikingaku Zasshi 1991;46:839-45.

https://doi.org/10.3412/jsb.46.839

Peng Q, Huang Y, Hou B, Hua D, Yao F, Qian Y. Green tea extract weakens the antibacterial effect of amoxicillin in methicillin-resistant Staphylococcus aureus infected mice. Phytother Res 2010;24:141-5.

https://doi.org/10.1002/ptr.2952

Sasaki H, Matsumoto M, Tanaka T, Maeda M, Nakai M, Hamada S, et al. Antibacterial activity of polyphenol components in oolong tea extract against Streptococcus mutans. Caries Res 2004;38:2-8.

https://doi.org/10.1159/000073913

Turkmen N, Velioglu YS, Sari F, Polat G. Effect of extraction conditions on measured total polyphenol contents and antioxidant and antibacterial activities of black tea. Molecules 2007;12:484-96.

https://doi.org/10.3390/12030484

Khan MI, Ahhmed A, Shin JH, Baek JS, Kim MY, Kim JD. Green tea seed isolated saponins exerts antibacterial effects against various strains of gram positive and gram negative bacteria, a comprehensive study in vitro and in vivo. Evid Based Complement Alternat Med 2018;2018:3486106.

https://doi.org/10.1155/2018/3486106

Ardakani MR, Golmohammadi S, Ayremlou S, Taheri S, Daneshvar S, Meimandi M. Antibacterial effect of Iranian green-tea-containing mouthrinse vs chlorhexidine 0.2%: an in vitro study. Oral Health Prev Dent 2014;12:157-62.

Shahid U, I, Butola BS, Roy A. Chitosan polysaccharide as a renewable functional agent to develop antibacterial, antioxidant activity and colourful shades on wool dyed with tea extract polyphenols. Int J Biol Macromol 2018;120:1999-2006. https://doi.org/10.1016/j.ijbiomac.2018.09.167

Amarowicz R, Pegg RB, Bautista DA. Antibacterial activity of green tea polyphenols against Escherichia coli K 12. Nahrung 2000;44:60-2.

https://doi.org/10.1002/(SICI)1521-3803(20000101)44:1<60::AID-FOOD60>3.0.CO;2-L

Ansari F, Pourjafar H, Kangari A, Homayouni A. Evaluation of the glucuronic acid production and antibacterial properties of Kombucha black tea. Curr Pharm Biotechnol 2019;20:985-90.

https://doi.org/10.2174/1389201020666190717100958

Najafi-Taher R, Ghaemi B, Amani A. Delivery of adapalene using a novel topical gel based on tea tree oil nano-emulsion: Permeation, antibacterial and safety assessments. Eur J Pharm Sci 2018;120:142-51.

https://doi.org/10.1016/j.ejps.2018.04.029

Cui H, Bai M, Lin L. Plasma-treated poly (ethylene oxide) nanofibers containing tea tree oil/betacyclodextrin inclusion complex for antibacterial packaging. Carbohydr Polym 2018;179:360-9.

https://doi.org/10.1016/j.carbpol.2017.10.011

Bashir S, Khan BM, Babar M, Andleeb S, Hafeez M, Ali S, et al. Assessment of bioautography and spot screening of TLC of green tea (Camellia) plant extracts as antibacterial and antioxidant agents. Indian J Pharm Sci 2014;76:364-70.

Jayaramudu T, Varaprasad K, Raghavendra GM, Sadiku ER, Mohana RK, Amalraj J. Green synthesis of tea Ag nanocomposite hydrogels via mint leaf extraction for effective antibacterial activity. J Biomater Sci Polym Ed 2017;28:1588-602. https://doi.org/10.1080/09205063.2017.1338501

Jazani NH, Shahabi S, Ali AA. Antibacterial effects of water soluble green tea extracts on multi-antibiotic resistant isolates of Pseudomonas aeruginosa. Pak J Biol Sci 2007;10:1544-6.

https://doi.org/10.3923/pjbs.2007.1544.1546

Tea polyphenols extracted from fresh young tea leaves exhibits antibacterial activity against some bacterial species: An in vitro study

Authors

Keywords:

Abstract

Downloads

References

Downloads

Published

How to Cite

Issue

Section

License

Similar Articles

Support by

lnfo

INDEXED IN

visitors