Total phenolics, total flavonoid, and total antioxidant capacity of medicinal plant-derived beverage: HydroZitLa

Authors

  • Nalinthip Lordumrongkiat Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Nattida Chotechuang Faculty of Science, Chulalongkorn University, Bangkok, Thailand
  • Natcha Madared Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Depicha Jindatip Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Chanchai Boonla Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

Keywords:

Oxidative stress, antioxidants, HydroZitLa, citrate, urinary stone

Abstract

Background: Antioxidants from natural sources are well-accepted for reducing oxidative damage and preventing the development of oxidative stress-related diseases. It is known that urinary stone development involves overproduction of reactive oxygen species and accumulation of oxidative injury. Supplements with antioxidants had been shown to inhibit kidney stone formation in animal models.

Objective: We developed a new beverage, called HydroZitLa (concentrate in pouch) to be used for prevention of urinary stone formation. HydroZitLa consisted of citrate (16 mEq) and naturally occurring antioxidants from Thai medicinal plants.

Methods: We investigated the total phenolic content (TPC), total flavonoid content (TFC), and total antioxidant capacity (TAC) in HydroZitLa. Also, cytotoxicity of HydoZitLa in HK-2 cells was evaluated.

Results: TPC and TFC in HydroZitLa were 10.2 gif.latex?\pm 0.5 mg galic acid equivalents (GAE) pouch and 2.8 gif.latex?\pm 0.2 mg catechin equivalent (CE) pouch, respectively. TAC in HydroZitLa determined by 2,2'-azino-bis (3-ethylbenzthiazoline- 6-sulphonic acid)(ABTS) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assays were 12.6 gif.latex?\pm 0.2 and 11.3 gif.latex?\pm 0.3 mg vitamin C equivalent antioxidation capacity (VCEAC) pouch, respectively. Stability test data at day 4 revealed that TPC and TAC (by DPPH) in HydroZitLa kept at 4oC were significantly higher than room temperature. Cytotoxicity test showed that HydroZitLa at 10% (v/v) and higher concentrations significantly caused reduction of HK-2 cell viability, and IC50 of HydroZitLa was of 24.6% (v/v).

Conclusion: TPC, TFC and TAC in HydroZitLa were considerably high. Natural antioxidants together with citrate in HydroZitLa had a potential to prevent the formation of urinary calculi. Further pre-clinical and clinical studies should be conducted to warrant the therapeutic action of HydroZitLa in urolithiasis.

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Published

2023-08-03

How to Cite

1.
Lordumrongkiat N, Chotechuang N, Madared N, Jindatip D, Boonla C. Total phenolics, total flavonoid, and total antioxidant capacity of medicinal plant-derived beverage: HydroZitLa. Chula Med J [Internet]. 2023 Aug. 3 [cited 2024 Oct. 8];64(4). Available from: https://he05.tci-thaijo.org/index.php/CMJ/article/view/253