Comparison of mitragynine stability in human blood in common blood collection tubes

Authors

  • Udomsak Hoonwijit Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Kanok Waithum Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Nat Tansrisawad Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

Keywords:

Mitragynine, stability in human blood, comparison, blood collection tube

Abstract

Background: Kratom (Mitragyna speciosa) is a plant commonly found in Southeast Asia. Kratom is one of the most abused substances as “a legal psychoactive drug or substance”. The main addictive substance of kratom is mitragynine. In forensic toxicology, one of the common problems is that there is no insightful information on mitragynine stability in human blood, so the study of the mitragynine stability would lead to the proper storage of the specimen containing mitragynine.

Objective: To study mitragynine stability in human blood in common blood collection tubes.

Methods: Mitragynine standard was spiked into the blood from the blood bank to get the sample concentration of 500 ng/mL. The sample was divided into 3 groups of common blood collection tubes which were sodium fluoride/potassium oxalate tube, Ethylenediaminetetraacetic acid tube and no anticoagulant tube. The concentrations of mitragynine were then measured and recorded in the conditions after 1 day, 3 days, 5 days, 7 days, 14 days and 30 days.

Results: Comparing the mean concentration of mitragynine with the elapsed time in each tube, it was found that there was no statistical significance (P > 0.05) among the blood collection tubes. However, it was found that the mitragynine concentration in sodium fluoride/potassium oxalate tube was more stable than the others. The difference was statistical significance (P < 0.05) when the concentrations of mitragynine on day 1 and day 14 were compared in sodium fluoride/potassium oxalate tube while the others revealed the decreasing mitragynine concentration between day 1 and day 7.

Conclusion: This study demonstrated the mitragynine stability in blood in the common blood collection tubes and revealed that the sodium fluoride/potassium oxalate tube was the appropriate collection tube. The limitation of the use of sodium fluoride/potassium oxalate tube to store blood sample for mitragynine determination was about 7 days.

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References

European Monitoring Centre for Drugs and Drug Addiction. EMCDDA-Europol 2012 Annual report on the implementation of council decision 2005/387/JHA [Internet]. 2013 [cited 2019 Jul 14]; Available from: http://www.emcdda.europa.eu/publications/implementation-reports/2012_en

United Nations Office on Drug and Crime. UNODC annual appeal 2017 [Internet]. [cited 2019 Jul 14]; Available from: https://www.unodc.org/unodc/en/ about-unodc/annual-report.html

Thanyarak Hospital statistics of drug abusers at Thanyarak Therapy Center for 2017 and 2018 fiscal years [Internet]. [cited 2019 Jul 14];Available from: http://www.pmnidat.go.th/thai/index.php?option= com_content&task=view&id=2738&Itemid=53

Feng LY, Battulga A, Han E, Chung H, Li JH. New psychoactive substances of natural origin: A brief review. J Food Drug Anal 2017;25:461-71. https://doi.org/10.1016/j.jfda.2017.04.001

Warner ML, Kaufman NC, Grundmann O. The pharmacology and toxicology of kratom: from traditional herb to drug of abuse. Int J Legal Med 2016;130:127-38.

https://doi.org/10.1007/s00414-015-1279-y

Lydecker AG, Sharma A, McCurdy CR, Avery BA, Babu KM, Boyer EW. Suspected adulteration of commercial kratom products with 7-hydroxymitragynine. J Med Toxicol 2016;12:341-9.

https://doi.org/10.1007/s13181-016-0588-y

Holler JM, Vorce SP, McDonough-Bender PC, Magluilo J Jr, Solomon CJ, Levine B. A drug toxicity death involving propylhexedrine and mitragynine. J Anal Toxicol 2011;35:54-9.

https://doi.org/10.1093/anatox/35.1.54

Neerman MF, Frost RE, Deking J. A drug fatality involving Kratom. J Forensic Sci 2013;58:278-9.

https://doi.org/10.1111/1556-4029.12009

McIntyre IM, Trochta A, Stolberg S, Campman SC. Mitragynine 'Kratom' related fatality: a case report with postmortem concentrations. J Anal Toxicol 2015;39:152-5. https://doi.org/10.1093/jat/bku137

Parthasarathy S, Ramanathan S, Ismail S, Adenan MI, Mansor SM, Murugaiyah V. Determination of mitragynine in plasma with solid-phase extraction and rapid HPLC-UV analysis, and its application to a pharmacokinetic study in rat. Anal Bioanal Chem 2010;397:2023-30.

https://doi.org/10.1007/s00216-010-3707-7

Kowalczuk AP, Lozak A, Zjawiony JK. Comprehensive methodology for identification of Kratom in police laboratories. Forensic Sci Int 2013; 233:238-43. https://doi.org/10.1016/j.forsciint.2013.09.016

Fu H, Cid F, Dworkin N, Cocores J, Shore G. Screening and Identification of Mitragynine and 7-Hydroxymitragynine in Human Urine by LC-MS/MS. Chromatography 2015; 2:253-64.

https://doi.org/10.3390/chromatography2020253

Scientific Working Group for Forensic Toxicology (SWGTOX) standard practices for method validation in forensic toxicology. J Anal Toxicol 2013;37:452-74. https://doi.org/10.1093/jat/bkt054

Major RE. QuEChERS - A new technique for multiresidue analysis of pesticides in foods and agricultural samples. LCGC Asia Pacific 2008;11:1-7.

Papsum DM, Chan-Hosokawa A, Friederich L, Brower J, Graf K, Logan B. The trouble with kratom: Analytical and interpretative issues involving mitragynine. J Anal Toxicol 2019;43:615-29.

https://doi.org/10.1093/jat/bkz064

Busardo FP, Kyriakou C, Tittarelli R, Mannocchi G, Pantano F, Santurro A, et al. Assessment of the stability of mephedrone in ante-mortem and postmortem blood specimens. Forensic Sci Int 2015;256:28-37. https://doi.org/10.1016/j.forsciint.2015.07.021

Dinis-Oliveira RJ, Viera DN, Magalhaes T. Guidelines for collection of biological samples for clinical and forensic toxicological analysis. Forensic Sci Res 2016;1:42-51.

https://doi.org/10.1080/20961790.2016.1271098

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Published

2023-08-03

How to Cite

1.
Hoonwijit U, Waithum K, Tansrisawad N. Comparison of mitragynine stability in human blood in common blood collection tubes. Chula Med J [Internet]. 2023 Aug. 3 [cited 2024 Oct. 8];64(4). Available from: https://he05.tci-thaijo.org/index.php/CMJ/article/view/267