Preparation and characterization of demineralized bone matrix/chitosan composite scaffolds for bone tissue engineering

Authors

  • Panudda Dechwongya Faculty of Pharmacy, Mahidol University, Bangkok, Thailand and Faculty of Pharmacy, Payap University, Chiangmai, Thailand
  • Korbtham Sathirakul Faculty of Pharmacy, Mahidol University, Bangkok, Thailand
  • Baldur Kristjánsson Faculty of Life and Environmental Sciences, University of Iceland, Reykjavík, Iceland
  • Suwabun Chirachanchai The Petroleum and Petrochemical College, Chulalongkorn University, Bangkok, Thailand
  • Sittisak Honsawek Faculty of Medicine, Chulalongkorn University, King Chulalongkorn Memorial Hospital, Bangkok, Thailand

Keywords:

Chitosan, demineralized bone matrix, human periosteal cells, scaffolds

Abstract

Background: A propitious alternative to supply bone substitutes is to develop living tissue substitutes based on biodegradable materials. Demineralized bone matrix (DBM) can support and promote osteogenesis; scaffold is also attractive for use in bone tissue engineering. Chitosan scaffold has been shown to possess biological and mechanical properties suitable for tissue engineering and clinical applications.

Objectives: This study aimed to develop a novel DBM/chitosan composite scaffold and to investigate whether or not it has the ability to support the attachment and proliferation of human periosteal cells in vitro for bone tissue engineering.

Methods: Chitosan and DBM/chitosan scaffolds (ratios 1:1 and 1:2) were fabricated with a low-cost, freeze-drying technique via thermally induced phase separation. The microstructure, mechanical performance, and biological activity of the scaffolds were studied. Scanning electron microscopy was employed to monitor the surface variation of chitosan and DBM/chitosan porous scaffolds.

Results: Both scaffolds had porosities and pore sizes between 80 and 250 microns. The compressive modulus of DBM/chitosan composite scaffolds was significantly higher than chitosan scaffolds. Growth of cells on 1:1 and 1:2 DBM/chitosan scaffolds had similar patterns throughout the cell-culture period and was significantly higher than that on chitosan scaffold on culture-day 14. The DBM/chitosan scaffolds have been developed with adequate pore structure and mechanical properties to serve as a support for periosteal cell growth.

Conclusion: DBM/chitosan composite scaffolds have mechanical properties and porosity sufficient to support ingrowth of new bone tissue. Cell attachment and proliferation findings indicate that DBM/chitosan composite scaffolds may be used as promising materials for bone tissue engineering application.

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References

Hanworawong A, Bunaprasert T. The efficacy of dermal extracted-bone powder scaffold on the healing of rat's calvarial bone defects. Chula Med J 2012;56:87-100.

Honsawek S, Powers RM, Wolfinbarger L. Extractable bone morphogenetic protein and correlation with induced new bone formation in an in vivo assay in the athymic mouse model. Cell Tissue Bank 2005;6:13-23. https://doi.org/10.1007/s10561-005-1445-4

Wildemann B, Kadow-Romacker A, Haas NP, Schmidmaier G. Quantification of various growth factors in different demineralized bone matrix preparations. J Biomed Mater Res A 2007;81:437-42.

https://doi.org/10.1002/jbm.a.31085

Katz JM, Nataraj C, Jaw R, Deigl E, Bursac P. Demineralized bone matrix as an osteoinductive biomaterial and in vitro predictors of its biological potential. J Biomed Mater Res B Appl Biomater 2009;89:127-34. https://doi.org/10.1002/jbm.b.31195

Boyan BD, Ranly DM, Schwartz Z. Use of growth factors to modify osteoinductivity of demineralized bone allografts: lessons for tissue engineering of bone. Dent Clin North Am 2006;50:217-28.

https://doi.org/10.1016/j.cden.2005.11.007

Ferretti C, Ripamonti U, Tsiridis E, Kerawala CJ, Mantalaris A, Heliotis M. Osteoinduction: translating preclinical promise into clinical reality. Br J Oral Maxillofac Surg 2010;48:536-9.

https://doi.org/10.1016/j.bjoms.2009.08.043

Nauth A, Giannoudis PV, Einhorn TA, Hankenson KD, Friedlaender GE, Li R, et al. Growth factors: beyond bone morphogenetic proteins. J Orthop Trauma 2010;24:543-6.

https://doi.org/10.1097/BOT.0b013e3181ec4833

Turnbull G, Clarke J, Picard F, Riches P, Jia L, Han F, et al. 3D bioactive composite scaffolds for bone tissue engineering. Bioact Mater 2017;3:278-314. https://doi.org/10.1016/j.bioactmat.2017.10.001

Di MA, Sittinger M, Risbud MV. Chitosan: a versatile biopolymer for orthopaedic tissue-engineering. Biomaterials 2005;26:5983-90. https://doi.org/10.1016/j.biomaterials.2005.03.016

Kim CH, Park SJ, Yang DH, Chun HJ. Chitosan for tissue engineering. Adv Exp Med Biol 2018;1077:475-85. https://doi.org/10.1007/978-981-13-0947-2_25

Seo YJ, Lee JY, Park YJ, Lee YM, Young K, Rhyu IC, et al. Chitosan sponges as tissue engineering scaffolds for bone formation. Biotechnol Lett 2004;26:1037-41.

https://doi.org/10.1023/B:BILE.0000032962.79531.fd

Keller L, Regiel-Futyra A, Gimeno M, Eap S, Mendoza G, Andreu V, et al. Chitosan-based nanocomposites for the repair of bone defects. Nanomedicine 2017;13:2231-40.

https://doi.org/10.1016/j.nano.2017.06.007

Adekogbe I, Ghanem A. Fabrication and characterization of DTBP-crosslinked chitosan scaffolds for skin tissue engineering. Biomaterials 2005;26:7241-50. https://doi.org/10.1016/j.biomaterials.2005.05.043

Li Z, Ramay HR, Hauch KD, Xiao D, Zhang M. Chitosan-alginate hybrid scaffolds for bone tissue engineering. Biomaterials 2005;26:3919-28. https://doi.org/10.1016/j.biomaterials.2004.09.062

Liu M, Zheng H, Chen J, Li S, Huang J, Zhou C. Chitosan-chitin nanocrystal composite scaffolds for tissue engineering. Carbohydr Polym 2016;152:832-40. https://doi.org/10.1016/j.carbpol.2016.07.042

Honsawek S, Bumrungpanichthaworn P, Thanakit V, Kunrangseesomboon V, Muchmee S, Ratprasert S, et al. Osteoinductive potential of small intestinal submucosa/demineralized bone matrix as composite scaffolds for bone tissue engineering. Asian Biomed 2010;4:913-22.

https://doi.org/10.2478/abm-2010-0119

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63. https://doi.org/10.1016/0022-1759(83)90303-4

O'Brien FJ, Harley BA, Yannas IV, Gibson L. Influence of freezing rate on pore structure in freeze-dried collagen-GAG scaffolds. Biomaterials 2004 ;25:1077-86. https://doi.org/10.1016/S0142-9612(03)00630-6

Ahsan SM, Thomas M, Reddy KK, Sooraparaju SG, Asthana A, Bhatnagar I. Chitosan as biomaterial in drug delivery and tissue engineering. Int J Biol Macromol 2018;110:97-109.

https://doi.org/10.1016/j.ijbiomac.2017.08.140

Tian M, Yang Z, Kuwahara K, Nimni ME, Wan C, Han B. Delivery of demineralized bone matrix powder using a thermogelling chitosan carrier. Acta Biomater 2012;8:753-62.

https://doi.org/10.1016/j.actbio.2011.10.030

Yan Z, Ruixin L, Yubo F, Hao L, Yong G, Liang W, et al. Microarchitectural and mechanical characterization of chitosan/hydroxyapatite/demineralized bone matrix composite scaffold. J Porous Mater 2012;19:251-9. https://doi.org/10.1007/s10934-011-9467-8

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Published

2023-08-15

How to Cite

1.
Dechwongya P, Sathirakul K, Kristjánsson B, Chirachanchai S, Honsawek S. Preparation and characterization of demineralized bone matrix/chitosan composite scaffolds for bone tissue engineering. Chula Med J [Internet]. 2023 Aug. 15 [cited 2024 Oct. 12];63(2). Available from: https://he05.tci-thaijo.org/index.php/CMJ/article/view/289