Resistant starch isolated from Luem-Pua glutinous rice decreases adipocyte size of visceral fat and thickness of thoracic aorta in high-fat diet-fed rats

Authors

  • Siwaporn Praman School of Medicine, Mae Fah Luang University.
  • Arunothai Wanta School of Medicine, Mae Fah Luang University.
  • Thaneeya Hawiset School of Medicine, Mae Fah Luang University.
  • Natthiya Sakulsak Faculty of Medical Science, Naresuan University.
  • Siam Popluechai Human gut microbiome for health research unit, School of Science, Mae Fah Luang University.
  • Keerakarn Somsuan School of Medicine, Mae Fah Luang University.

Keywords:

Luem-Pua glutinous rice, resistant starch, visceral fat, thoracic aorta, high-fat diet

Abstract

Background : Luem-Pua glutinous rice has been reported to contain a high content of resistant starch (RS). Previous studies showed weight loss, decreased blood glucose and lipid profiles levels, as well as decreased hyperplasia and hypertrophy of adipocytes in RS treatment. However, the study of protective effects of RS isolated from Luem-Pua glutinous rice on obesity is still needed to provide supportive information for potential use as dietary supplements or development of therapeutic agents for the treatment obesity.

Objective : We aimed to investigate the effects of RS isolated from Luem-Pua glutinous rice on body weight and visceral fat weight, blood parameters, histological changes of visceral fat and thoracic aorta in high-fat diet-fed rats.

Methods : Eighteen male rats were divided into three groups (n = 6/group) and daily treated as follows: control, normal diet; HF+DW, a high-fat diet with distilled water; HF+LP, a high-fat diet with RS isolated from Luem-Pua glutinous rice. We investigated food intake (FI), final body weight (FBW) and length (FBL), serum glucose, serum lipid profiles. In addition, histology of visceral fat and thoracic aorta were investigated by hematoxylin and eosin (H&E) staining at the end of the experiment.

Results : FI and FBW showed significantly negative correlation in the HF group, especially in HF+LP. FBW and visceral fat weight of HF+LP and HF+DW were significantly increased in comparison to control group. FBL and all blood profiles were not significantly different among the three groups. Ratio of adipocyte area to number and thickness of the thoracic aorta were significantly reduced in HF+LP in comparison to control and HF+DW groups

Conclusion : This study addressed specific protective effects of RS isolated from Luem Pua glutinous rice on obesity as follows: 1) reduction of adipocytes size, 2) prevention of pathological changes of thoracic aorta, and 3) decreasing of thoracic aorta thickness in high-fat diet-fed rats.

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References

Diaz ME, Jimenez S, Garcia RG, Bonet M, Wong I. Overweight, obesity, central adiposity and associated chronic diseases in cuban adults. MEDICC Rev 2009;11:23-8.

https://doi.org/10.37757/MR2009V11.N4.7

Mozaffarian D, Hao T, Rimm EB, Willett WC, Hu FB. Changes in diet and lifestyle and longterm weight gain in women and men. N Engl J Med 2011;364:2392-404.

https://doi.org/10.1056/NEJMoa1014296

Shoelson SE, Herrero L, Naaz A. Obesity, inflammation, and insulin resistance. Gastroenterology 2007;132:2169-80. https://doi.org/10.1053/j.gastro.2007.03.059

Hill JO, Melanson EL, Wyatt HT. Dietary fat intake and regulation of energy balance: implications for obesity. J Nutr 2000;130(2S Suppl): 284S-8S. https://doi.org/10.1093/jn/130.2.284S

Wang YC, McPherson K, Marsh T, Gortmaker SL, Brown M. Health and economic burden of the projected obesity trends in the USA and the UK. Lancet 2011;378:815-25.

https://doi.org/10.1016/S0140-6736(11)60814-3

Buettner R, Scholmerich J, Bollheimer LC. Highfat diets: modeling the metabolic disorders of human obesity in rodents. Obesity (Silver Spring) 2007;15:798-808. https://doi.org/10.1038/oby.2007.608

Pramai P, Jiamyangyuen S. Chemometric classification of pigmented rice varieties based on antioxidative properties in relation to color. Songklanakarin J Sci Technol 2016;38:463-72.

Popluechai S, Laosat N, Kitiraj S, Sasanatayart R, Kespechara K. The combination of GBBSIGPT1 haplotype of some Thai rice in relation to retrograde resistant strach [Internet]. 2013 [cited 2018 Apr 13]. Avialable from: http://www.thaiscience.info/Article%20for%20 ThaiScience/Article/2/10030480.pdf

Birt DF, Boylston T, Hendrich S, Jane JL, Hollis J, Li L, et al. Resistant starch: promise for improving human health. Adv Nutr 2013;4:587-601. https://doi.org/10.3945/an.113.004325

Englyst HN, Cummings JH. Digestion of the polysaccharides of some cereal foods in the human small intestine. Am J Clin Nutr 1985;42:778-87. https://doi.org/10.1093/ajcn/42.5.778

Belobrajdic DP, King RA, Christophersen CT, Bird AR. Dietary resistant starch dosedependently reduces adiposity in obesityprone and obesity-resistant male rats. Nutr Metab (Lond) 2012;9:93.

https://doi.org/10.1186/1743-7075-9-93

Harazaki T, Inoue S, Imai C, Mochizuki K, Goda T. Resistant starch improves insulin resistance and reduces adipose tissue weight and CD11c expression in rat OLETF adipose tissue. Nutrition 2014;30:590-5. https://doi.org/10.1016/j.nut.2013.10.020

Park OJ, Kang NE, Chang MJ, Kim WK. Resistant starch supplementation influences blood lipid concentrations and glucose control in overweight subjects. J Nutr Sci Vitaminol (Tokyo) 2004;50:93-9.

https://doi.org/10.3177/jnsv.50.93

Bronkowska M, Orzel D, Lozna K, Styczynska M, Biernat J, Gryszkin A, et al. Effect of resistant starch RS4 added to the high-fat diets on selected biochemical parameters in Wistar rats. Rocz Panstw Zakl Hig 2013;64:19-24.

Ghibaudi L, Cook J, Farley C, van Heek M, Hwa JJ. Fat intake affects adiposity, comorbidity factors, and energy metabolism of spraguedawley rats. Obes Res 2002;10:956-63.

https://doi.org/10.1038/oby.2002.130

Johnston SL, Souter DM, Tolkamp BJ, Gordon IJ, Illius AW, Kyriazakis I, et al. Intake compensates for resting metabolic rate variation in female C57BL/6J mice fed highfat diets. Obesity (Silver Spring) 2007;15: 600-6. https://doi.org/10.1038/oby.2007.550

Ahima RS. Revisiting leptin's role in obesity and weight loss. J Clin Invest 2008;118:2380-3.

https://doi.org/10.1172/JCI36284

Morton GJ, Cummings DE, Baskin DG, Barsh GS, Schwartz MW. Central nervous system control of food intake and body weight. Nature 2006;443:289-95. https://doi.org/10.1038/nature05026

Ahima RS, Prabakaran D, Mantzoros C, Qu D, Lowell B, Maratos-Flier E, et al. Role of leptin in the neuroendocrine response to fasting. Nature 1996;382:250-2.

https://doi.org/10.1038/382250a0

Okosun IS, Chandra KM, Choi S, Christman J, Dever GE, Prewitt TE. Hypertension and type 2 diabetes comorbidity in adults in the United States: risk of overall and regional adiposity. Obes Res 2001;9:1-9.

https://doi.org/10.1038/oby.2001.1

Park SY, Kim YW, Kim JE, Kim JY. Age-associated changes in fat metabolism in the rat and its relation to sympathetic activity. Life Sci 2006; 79:2228-33. https://doi.org/10.1016/j.lfs.2006.07.014

Aziz AA, Kenney LS, Goulet B, Abdel-Aal el-S. Dietary starch type affects body weight and glycemic control in freely fed but not energy-restricted obese rats. J Nutr 2009; 139:1881-9.

https://doi.org/10.3945/jn.109.110650

Higgins JA, Jackman MR, Brown IL, Johnson GC, Steig A, Wyatt HR, et al. Resistant starch and exercise independently attenuate weight regain on a high fat diet in a rat model of obesity. Nutr Metab (Lond) 2011;8:49. https://doi.org/10.1186/1743-7075-8-49

Johnson PR, Hirsch J. Cellularity of adipose depots in six strains of genetically obese mice. J Lipid Res 1972;13:2-11. https://doi.org/10.1016/S0022-2275(20)39428-1

Kubota N, Terauchi Y, Miki H, Tamemoto H, Yamauchi T, Komeda K, et al. PPAR gamma mediates high-fat diet-induced adipocyte hypertrophy and insulin resistance. Mol Cell 1999;4:597-609.

https://doi.org/10.1016/S1097-2765(00)80210-5

Kabir M, Rizkalla SW, Champ M, Luo J, Boillot J, Bruzzo F, et al. Dietary amylose-amylopectin starch content affects glucose and lipid metabolism in adipocytes of normal and diabetic rats. J Nutr 1998;128:35-43. https://doi.org/10.1093/jn/128.1.35

Kabir M, Rizkalla SW, Quignard-Boulange A, Guerre-Millo M, Boillot J, Ardouin B, et al. A high glycemic index starch diet affects lipid storage-related enzymes in normal and to a lesser extent in diabetic rats. J Nutr 1998;128: 1878-83. https://doi.org/10.1093/jn/128.11.1878

So PW, Yu WS, Kuo YT, Wasserfall C, Goldstone AP, Bell JD, et al. Impact of resistant starch on body fat patterning and central appetite regulation. PLoS One 2007;2:e1309.

https://doi.org/10.1371/journal.pone.0001309

Ferlosio A, Arcuri G, Doldo E, Scioli MG, De Falco S, Spagnoli LG, et al. Age-related increase of stem marker expression influences vascular smooth muscle cell properties. Atherosclerosis 2012;224:51-7.

https://doi.org/10.1016/j.atherosclerosis.2012.07.016

Kamel EO, Gad El-rab WM. The effect of ginger on experimentally induced atherosclerosis in the aorta of rabbits: a histological and immunohistochemical study. Al-Azhar Assiut Med J 2017;15:43-51.

https://doi.org/10.4103/AZMJ.AZMJ_3_17

Arad M, Seidman CE, Seidman JG. AMP-activated protein kinase in the heart: role during health and disease. Circ Res 2007;100:474-88. https://doi.org/10.1161/01.RES.0000258446.23525.37

Boon CM, Ng MH, Choo YM, Mok SL. Super, red palm and palm oleins improve the blood pressure, heart size, aortic media thickness and lipid profile in spontaneously hypertensive rats. PLoS One 2013;8:e55908. https://doi.org/10.1371/journal.pone.0055908

Santana AB, Souza Oliveira TC, Bianconi BL, Barauna VG, Santos EW, Alves TP, et al. Effect of high-fat diet upon inflammatory markers and aortic stiffening in mice. Biomed Res Int 2014;2014:914102.

https://doi.org/10.1155/2014/914102

Sel uk EB, Sungu M, Parlakpinar H, Ermi N, Tasl1dere E, Vard1 N, et al. Evaluation of the cardiovascular effects of varenicline in rats. Drug Des Devel Ther 2015;9:5705-17.

https://doi.org/10.2147/DDDT.S92268

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Published

2023-08-16

How to Cite

1.
Praman S, Wanta A, Hawiset T, Sakulsak N, Popluechai S, Somsuan K. Resistant starch isolated from Luem-Pua glutinous rice decreases adipocyte size of visceral fat and thickness of thoracic aorta in high-fat diet-fed rats. Chula Med J [Internet]. 2023 Aug. 16 [cited 2024 Nov. 6];62(3). Available from: https://he05.tci-thaijo.org/index.php/CMJ/article/view/337