Exploring the therapeutic potential of Trigonella foenumgraecum extract-loaded transferosomal gel in complete Freund’s adjuvant-induced rat models

Vandana; Hema  Arya; Koushal  Dhamija; Preeti  Singh; Gautam  Kumar

Authors

Vandana Sharda University, Knowledge Park III, Greater Noida, Uttar Pradesh, India
Hema Arya Sharda University, Knowledge Park III, Greater Noida, Uttar Pradesh, India
Koushal Dhamija Lloyd Institute of Management and Technology, Knowledge Park II, Greater Noida, Uttar Pradesh, India
Preeti Singh Sharda University, Knowledge Park III, Greater Noida, Uttar Pradesh, India
Gautam Kumar Apeejay Stya University, Sohna-Palwal Road, Sohna, Gurugram, Haryana, India

Keywords:

Complete Freund’s adjuvant-induced rat models, rheumatoid arthritis, topical drug delivery system, transferosomal gel, Trigonella foenum-graecum

Abstract

Background: Rheumatoid arthritis (RA) is a debilitating autoimmune disorder that is characterized by inflammation and joint damage.

Objective: This study evaluated the antiarthritic activity of Trigonella foenum-graecum (TFG) transferosomal gel in a Freund’s adjuvant-induced arthritic rat model and compared it with diclofenac gel, a standard antiinflammatory drug.

Methods: Arthritis was induced in rats by injecting 0.1 mL (1 mg/mL) of complete Freund’s adjuvant suspension into the subplantar region of the left hind foot, and they were allowed to develop arthritis for 21 days.

Results: Arthritis induction led to a considerable increase in paw volume, which is indicative of inflammation. Treatment with both TFG-transferosomal gel and diclofenac gel substantially reduced the paw volume, thus demonstrating their anti-inflammatory effects. In addition, the TFG-transferosomal gel reduced the arthritic scores, indicating its potential to ameliorate arthritic symptoms. Both treatments improved locomotor activity, which suggests relief from pain-induced hypoactivity. Furthermore, biochemical analysis revealed that the TFG-transferosomal gel effectively reduced inflammatory enzyme activity and mediator levels, comparable to that of diclofenac gel.

Conclusion: These results demonstrate the anti-inflammatory efficacy of the TFG-transferosomal gel, thus highlighting its potential as a therapeutic option for managing arthritis-associated inflammation and symptoms. Further research is warranted to elucidate its mechanisms of action and assess its clinical utility

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