Dose-dependent effect of local treatment of Phyllanthus emblica L. cream on diabetic wound

Authors

  • Ting-ting Liao Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Supakanda Sukpat Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Chaisak Chansriniyom Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand
  • Suthiluk Patumraj Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

Keywords:

Diabetic wound, Phyllanthus emblica Linn., wound closure, capillary vascularity

Abstract

Background: Non-healing diabetic ulcers are the most common cause of amputation. Several studies have reported for new, more effective treatments for chronic wounds in diabetic patients.

Objective: This study aimed to evaluate the effect of local treatment of Phyllanthus emblica Linn. (PE) on a full-thickness wound model in streptozotocin-induced diabetic mice.

Methods: Balb/c mice were divided into five groups: control group (CON), diabetic wounded group (DM, streptozotocin 45 mg/kg i.p. daily for 5 days), diabetic wounded group with daily treatment of different concentrations of PE cream (10%; 100%; and 200%, w/v). Seven days after the diabetic induction, all mice were created bilateral full-thickness excisional skin wounds on the back and received vehicle or PE cream into wound bed. At day 14 post-wounding, the percentage of wound closure (%WC) and the percentage of capillary vascularity (%CV) were determined by using confocal fluorescence microscopy and digital image analysis.

Results: The dose-dependent of PE on diabetic wounds were determined by both findings of %WC and %CV. The results showed positive correlation between various doses of topical PE and 14-day %CV post-wound creation (r = 0.7197) (P = 0.0017). The linear regression equation, Y = 0.01788X + 37.35, with R2 = 0.5180, described the relationship between PE doses and CV%.

Conclusion: These findings show that local administration of PE cream improved the healing process of diabetic wounds in associated with PE’s angiogenic effect in a dose-dependent manner.

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Published

2023-07-17

How to Cite

1.
Liao T- ting, Sukpat S, Chansriniyom C, Patumraj S. Dose-dependent effect of local treatment of Phyllanthus emblica L. cream on diabetic wound. Chula Med J [Internet]. 2023 Jul. 17 [cited 2024 Oct. 8];66(2). Available from: https://he05.tci-thaijo.org/index.php/CMJ/article/view/65