Change in cell viability, reactive oxygen species production and oxidative stress in older patients with coronary heart disease under going lifestyle management program.

Abstract

Objective : To study the effects of lifestyle management (LM) program on lipid profiles and oxidative stress markers in patients with coronary heart disease (CHD), and in vitro effect of sera patients on human coronary artery endothelial cells (HCAECs).

Methods : A total of 30 patients with CHD (mean age: 63.28 years) were randomized into two groups: LM-intervened and usual care (UC) groups. Lipid profiles, oxidized LDL and protein carbonyl were measured in collected blood specimens from the patients at baseline, 6 and 12 months. Using patients’ sera and HCAECs, we measured for cell viability by MTT [3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay and intracellular reactive oxygen species (ROS) production by fluorescence detection of 2,7-dichlorofluorescein (DCF). Dietary intake, frequency of exercise and quality of life were assessed by a self-report questionnaire and a qualityof- life questionnaire.

Results : After 6 months of intervention, serum total cholesterol, triglyceride, oxidized LDL and protein carbonyl levels were significantly decreased in the LM group but they did not improve at 12 months. In vitro, MTT assay indicated that HCAEC cell viability increased in the sera from the patients in LM group after intervention. Fluorometric detection of ROS production significantly decreased after 6 months of intervention in LM group but showed no significant change at 12 months in both groups. Patients in LM group significantly increased vegetables consumption in specified portion size (3 - 5 serving/day) and quality of life in social and economic factors after 12 months of intervention.

Conclusion : These findings indicate that the LM program decreases lipid profiles and oxidative stress as well as increases endothelial cell viability in patients with CHD. It is strongly recommended as an efficient strategy to decrease the risk of cardiovascular disease development.