One-step multiplex RT-PCR method for detection and serotyping of foot and mouth disease virus

Abstract

Background: Foot and Mouth Disease is a severe disease in double hoofed animals causing
economic impact. Early report of the serotypes is necessary for control and decrease the loss. Nowadays,
we use World Organization for Animal Health standard method (OIE standard method) for diagnosis
including ELISA typing and virus isolation. Disadvantage of ELISA typing is this method can done with
high amount of viral agents in the sample. When low amount of viral agents in the sample, it has to
increase the virus quantity using “virus isolation”, then re-confirm by the ELISA typing test. One-step
multiplex RT-PCR method had been developed, this method could identify the serotype of FMDV
within one process and could work with low quantity of virus.