Plant based extracts show novel sanitising effects against Escherichia coli on romaine lettuce and cucumber

Authors

  • Tinashe Mandimutsira Faculty of Science and Engineering, Bindura University Science Education, Zimbabwe
  • Paul Tirivanhu Njowa Faculty of Science Education, Bindura University Science Education, Zimbabwe
  • Constance Chingwaru Faculty of Science Education, Bindura University Science Education, Zimbabwe
  • Walter Chingwaru Faculty of Science Education, Bindura University Science Education, Zimbabwe

Keywords:

Cucumis sativa, Lactuca sativa, romaine lettuce, cucumber, diarrhoea, medicinal, inhibition, WASH

Abstract

Background: Consumption of ready-to-eat vegetables (RTEV) is increasing around the globe.

Objective: The current study sought to assess the efficacy of extracts of selected African medicinal plants [Lannea edulis (Sond.) Engl. var. Edulis (L. edulis), Cassia abbreviata Oliv. (C. abbreviata), Solanum incanum (S. incanum) and Grewia bicolor (G. bicolor)] on removal of E. coli, an indicator of faecal contamination from Lactuca sativa L. var. longifolia (L. sativa) (Romaine lettuce) leaves, and Cucumis sativa L. (C. sativa) (cucumber) surfaces.

Methods: Two cm2 square sections of lettuce and cucumber epidemal tissues were soaked in extracts of the medicinal plants (10 -1 of original extracts) for 1 hr. Washing of the RTEV with sterile water caused significant reductions in E. coli loads on lettuce or cucumber (P < 0.01) surfaces compared to unwashed controls.

Results: Extracts from the medicinal plants caused reductions in E. coli loads on all both RTEV (load reduction by at least 2 log colony forming units (CFU)/cm2) (P < 0.01) without changes in surface morphology and colour. Use of extracts from L. edulis, C. abbreviata, S. incanum and G. bicolor have potential for use in sanitisation of RTEV, especially in killing, inhibiting or removing pathogenic E. coli that are transmitted via these fresh foods and products. While washing with G. bicolor resulted in significant reductions of E. coli on cucumber and lettuce surfaces, its efficacy was lowest compared to the rest of the treatments; hence, it is the least favourable of the selected medicinal plants. Soaking in the extract preparations did not lead to significant changes in odor, texture or taste of the vegetables.

Conclusion: Use of extracts from these plants is suggested as a possible way to sanitise RTEV to reduce occurrence of, or contain outbreaks of diseases that are borne on these vegetables.

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References

Camino Feltes MM, Arisseto-Bragotto AP, Block JM. Food quality, food-borne diseases, and food safety in the Brazilian food industry. Food Qual Saf 2017;1:13-27. https://doi.org/10.1093/fqs/fyx003

Liu J, Gratz J, Maro A, Kumburu H, Kibiki G, Taniuchi M, et al. Simultaneous detection of six diarrheacausing bacterial pathogens with an in-house PCRluminex assay. J Clin Microbiol 2012;50:98-103.

https://doi.org/10.1128/JCM.05416-11

Jay-Russell MT, Hake AF, Bengson Y, Thiptara A, Nguyen T. Prevalence and characterisation of Escherichia coli and Salmonella strains isolated from stray dog and coyote feces in a major leafy greens production region at the United States-Mexico border. PLoS One 2014;9:1-14.

https://doi.org/10.1371/journal.pone.0113433

Scott RA, Thilmony R, Harden LA, Zhou Y, Brandl MT. Escherichia coli O157: H7 converts plant-derived choline to glycine betaine for osmoprotection during pre-and post-harvest colonisation of injured lettuce leaves. Front Microbiol 2017;8:1-14. https://doi.org/10.3389/fmicb.2017.02436

Slayton RB, Turabelidze G, Bennett SD, Schwensohn CA, Yaffee AQ, Khan F, et al. Outbreak of Shiga toxin-producing Escherichia coli (STEC) O157: H7 associated with romaine lettuce consumption, 2011. PLoS One 2013;8:1-6. https://doi.org/10.1371/journal.pone.0055300

Hurst WC. Safety aspects of fresh-cut fruits and vegetables. In Lamikanra O, editor. Fresh-cut fruits and vegetables: Science, technology, and market. Philadelphia, PA: Taylor & Francis; 2002.p.45-90.

https://doi.org/10.1201/9781420031874.ch4

Jung Y, Jang H, Matthews KR. Effect of the food production chain from farm practices to vegetable processing on outbreak incidence. Microb Biotechnol 2014;7:517-27.

https://doi.org/10.1111/1751-7915.12178

Nguyen B, Bauman A, Gale J, Banks E, Kritharides L, Ding D. Fruit and vegetable consumption and all-cause mortality: evidence from a large Australian cohort study. Int J Behav Nutr Phys Act 2016;13:9.

https://doi.org/10.1186/s12966-016-0334-5

Buck JW, Walcott RR, Beuchat LR. Recent trends in microbiological safety of fruits and vegetables. Plant Health Progress 2003;4:25. https://doi.org/10.1094/PHP-2003-0121-01-RV

Callejón RM, Rodríguez-Naranjo MI, Ubeda C, Hornedo-Ortega R, Garcia-Parrilla MC, Troncoso AM. Reported foodborne outbreaks due to fresh produce in the United States and European Union: trends and causes. Foodborne Pathog Dis 2015;12:32-8. https://doi.org/10.1089/fpd.2014.1821

Amoah P, Drechsel P, Abaidoo RC, Klutse A. Effectiveness of common and improved sanitary washing methods in selected cities of West Africa for the reduction of coliform bacteria and helminth eggs on vegetables. Trop Med Int Health 2007;12:40-50. https://doi.org/10.1111/j.1365-3156.2007.01940.x

Uhlig E, Olsson C, He J, Stark T, Sadowska Z, Molin G, et al. Effects of household washing on bacterial load and removal of Escherichia coli from lettuce and "ready to eat" salads. Food Sci Nutr 2017;5:1215-20. https://doi.org/10.1002/fsn3.514

Bachelli ML, Amaral RD, Benedetti BC. Alternative sanitisation methods for minimally processed lettuce in comparison to sodium hypochlorite. Braz J Microbiol 2013;44:673-8.

https://doi.org/10.1590/S1517-83822013005000065

Hood E. Tap water and trihalomethanes: Flow of concerns continues [abstract]. Environ Health Perspect 2005;113:A474. https://doi.org/10.1289/ehp.113-a474

Min SC, Roh SH, Niemira BA, Boyd G, Sites JE, Uknalis J, et al. In-package inhibition of E. coli O157:H7 on bulk Romaine lettuce using cold plasma. Food Microbiol 2017;65:1-6.

https://doi.org/10.1016/j.fm.2017.01.010

Maroyi A. Ximenia caffra Sond.(Ximeniaceae) in sub-Saharan Africa: A synthesis and review of its medicinal potential. J Ethnopharmacol 2016;184:81-100. https://doi.org/10.1016/j.jep.2016.02.052

Nejatzadeh-Barandozi F. Antibacterial activities and antioxidant capacity of Aloe vera. Org Med Chem Lett 2013;3:5. https://doi.org/10.1186/2191-2858-3-5

Hoet S, Pieters L, Muccioli GG, Habib-Jiwan JL, Opperdoes FR, Quetin-Leclercq J. Antitrypanosomal activity of triterpenoids and sterols from the leaves of Strychnos spinosa and related compounds. J Nat Prod 2007;70:1360-3. https://doi.org/10.1021/np070038q

Chivengwa C, Mandimutsira T, Gere J, Magogo C, Chikanza I, Vidmar J, et al. Inhibition of Escherichia coli and Salmonella spp. By traditional phytomedicines that are commonly used to treat gastroenteritis in Zimbabwe [abstract]. World Academy of Science, Engineering and Technology, Int J Pharmacol Pharmaceut Sci 2016;3:1.

Sobeh M, Mahmoud MF, Abdelfattah MA, Cheng H, El-Shazly AM, Wink M. A proanthocyanidin-rich extract from Cassia abbreviata exhibits antioxidant and hepatoprotective activities in vivo. J Ethnopharmacol 2018;213:38-47. https://doi.org/10.1016/j.jep.2017.11.007

Indhumathi T, Mohandass S. Efficacy of ethanolic extract of Solanum incanum fruit extract for its antimicrobial activity. International Journal of Current Microbiology and Applied Sciences 2014;3:939-49.

Sahle T, Okbatinsae G. Phytochemical investigation and antimicrobial activity of the fruit extract of Solanum incanum grown in Eritrea. Ornamental Med Plants 2017;1:15-25.

Jaspers MW, Bashir AK, Zwaving JH, Malingré TM. Investigation of Grewia bicolor juss. J Ethnopharmacol 1986;17:205-11. https://doi.org/10.1016/0378-8741(86)90109-1

Kao LS, Green CE. Analysis of variance: is there a difference in means and what does it mean?. J Surg Res 2008;144:158-70. https://doi.org/10.1016/j.jss.2007.02.053

Andrews JM. Determination of minimum inhibitory concentrations. J Antimicrob Chemother 2001;48 Suppl 1:5-16. https://doi.org/10.1093/jac/48.suppl_1.5

Turner L. Analysis of variance, one-way completely randomized. Online calculator [Internet]. 2018 [cited 2018 Apr 14]. Available from:

http://turner.faculty.swau.edu/mathematics/math241/materials/anova/.

Alves MJ, Ferreira IC, Froufe HJ, Abreu RM, Martins A, Pintado, M. Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies. J Appl Microbiol 2013;115:346-57. https://doi.org/10.1111/jam.12196

Borges A, Ferreira C, Saavedra MJ, Simoes M. Antibacterial activity and mode of action of ferulic and gallic acids against pathogenic bacteria. Microb Drug Resist 2013;19:256-65.

https://doi.org/10.1089/mdr.2012.0244

Lou Z, Wang H, Zhu S, Ma C, Wang Z. Antibacterial activity and mechanism of action of chlorogenic acid. J Food Sci 2011;76:1-7. https://doi.org/10.1111/j.1750-3841.2011.02213.x

Al-Marby A, Ejike CE, Nasim MJ, Awadh-Ali NA, AlBadani RA, Alghamdi GM, et al. Nematicidal and antimicrobial activities of methanol extracts of 17 plants, of importance in ethnopharmacology, obtained from the Arabian Peninsula. J Intercult Ethnopharmacol 2016;5:114-21.

https://doi.org/10.5455/jice.20160217040157

Beaman-Mbaya V, Muhammed SI. Antibiotic action of Solanum incanum Linnaeus. Antimicrob Agents Chemother 1976;9:920-4. https://doi.org/10.1128/AAC.9.6.920

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Published

2023-07-26

How to Cite

1.
Mandimutsira T, Tirivanhu Njowa P, Chingwaru C, Chingwaru W. Plant based extracts show novel sanitising effects against Escherichia coli on romaine lettuce and cucumber. Chula Med J [Internet]. 2023 Jul. 26 [cited 2024 May 20];64(2). Available from: https://he05.tci-thaijo.org/index.php/CMJ/article/view/188

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Vol. 64 No. 2 (2020): Chulalongkorn Medical Journal

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Original Articles

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