In vitro biocompatibility of novel titanium-based amorphous alloy thin film in human osteoblast-like cells

Authors

  • Saran Tantavisut Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Boonrat Lohwongwatana Faculty of Engineering, Chulalongkorn University, Bangkok, Thailand
  • Atchara Khamkongkaeo Faculty of Engineering, Chulalongkorn University, Bangkok, Thailand
  • Aree Tanavalee Chulalongkorn University, Bangkok, Thailand
  • Pairat Tangpornprasert Faculty of Engineering, Chulalongkorn University, Bangkok, Thailand
  • Pubul Ittiravivong Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

Keywords:

Titanium-based alloy, biocompatibility, toxicity, calcification

Abstract

Background: Toxic free Ti-based amorphous alloy has the potential to be used in biomedical fields due to its excellent biocompatibility and osseointegration.

Objectives: The purpose of this study was to develop a series of Ti44Zr10Pd10Cu6+ xCo23-xTa7 (x = 0, 4, 8) and examine their biocompatibility, biological properties, and toxicity in osteoblast-like cells.

Methods: Having developed the alloy ingots by induction melting, we used the cast rod as a plasma cathode in a filtered cathodic vacuum arc deposition chamber to coat a 25-nm thin film of amorphous alloy on cover glass slides. These coated cover glass slides were then examined for biocompatibility. The biocompatibility tests in SaOS2 osteoblast-like cells were performed using a methylthiazol tetrazolium assay and alizarin red staining. The medical grade Ti-6Al-4V alloys was studied in parallel as a control material.

Results: There was no statistically significant difference in number of living cells between all novel alloys compared with Ti-6Al-4V thin film. Alizarin red staining showed that all novel alloy thin film had significantly higher percentage area of calcification in comparison with Ti-6Al-4V thin film control (P < 0.05). In terms of calcification size, the Ti44Zr10Pd10Cu10Co19Ta7 and Ti44Zr10Pd10Cu14Co15Ta7 showed significantly greater calcification than the control (P < 0.05) while Ti44Zr10Pd10Cu6Co23Ta7 also demonstrated larger calcification in comparison with control but no statistical significance (P = 0.27).

Conclusion: The results indicated that all investigated Ti-based alloys were found to be non-cytotoxic and support differentiation of osteoblast-like cells.

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Published

2023-08-15 — Updated on 2023-11-20

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How to Cite

1.
Tantavisut S, Lohwongwatana B, Khamkongkaeo A, Tanavalee A, Tangpornprasert P, Ittiravivong P. In vitro biocompatibility of novel titanium-based amorphous alloy thin film in human osteoblast-like cells. Chula Med J [Internet]. 2023 Nov. 20 [cited 2024 Nov. 22];63(2). Available from: https://he05.tci-thaijo.org/index.php/CMJ/article/view/282

Issue

Vol. 63 No. 2 (2019): Chulalongkorn Medical Journal

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Original Articles

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