Antioxidant effects of unpolished Sung-Yod sticky rice on prevention of ethylene glycol-induced renal pathology in rats
Keywords:
Sung-Yod sticky rice, antioxidant, ethylene glycol, renal pathologyAbstract
Background : Kidney stone is a chronic disease that is found worldwide. The high prevalence of the kidney stone in Thailand observed in the northeastern part which was about 16.9%. The pathogenesis of calcium oxalate stone formation originated from crystallization of calcium and oxalate ions in renal tubular fluid, overgrowth, aggregation and adhesion to renal epithelial cells. The adhesion of CaOx crystals to renal tubular cell is a critical step of kidney stone formation. It can cause increased oxidative stress and result in the renal tubular cells damage. Studying in natural products may be an alternative way to prevent the pathogenesis of stone formation.
Objective : To study the antioxidant potential of Sung Yod sticky rice extract on the prevention of ethylene glycol-induced renal tissue damage in rats.
Methods : Unpolished Sung-Yod sticky rice was extracted. The total anthocyanin and phenolic contents were determined by pH differential method and Follin Ciocalteu reagent assay, respectively. The percentage of antioxidant activity was assessed by DPPH scavenging assay compared with the standard serial concentrations of ascorbic acid. In in vivo experiment, rats were divided into two groups: ethylene glycol control (EGC) group received normal drinking water for two weeks and SY treated group was daily fed SY at the dose of 200 mg/kg by gastric intubation for two weeks. After two weeks, 0.5% ethylene glycol was administered in all groups, and 1% ammonium chloride was fed by gastric intubation for one week. At the end of the experiment, the kidney tissues were collected for histological study. Oxidative stress, 8 hydroxyguanosine (8-OHG), was determined by immunoperoxidase whereas antioxidant biomarkers including superoxide dismutase (SOD) and catalase were observed by Western immunoblotting.
Results : The mean total anthocyanin and total phenolic compounds were 19.566 g/g dry weights and 0.295 mg/g dry weight, respectively. The half maximal inhibitory concentration of the Sung-Yod sticky rice extract was significantly increased at the fifth teen folded compared with ascorbic acid (P < 0.05). Pathological changes of the kidney including tubular dilation, shrinkage of glomerulus and the flattened renal tubular cells in rat were reduced by treatment with Sung Yod sticky rice extract. The number of 8-OHG -positive cells decreased in the SY treated group. The expression of SOD and catalase significantly increased in the SY treated group, as compared with EGC group.
Conclusion : These findings demonstrate that the Sung-Yod sticky rice extract has a protective effect on the ethylene glycol-induced renal pathology. This extract might be beneficial in preventing the cause of kidney stone.
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Li YH, Yu SL, Gan XG, Pan SH, Teng YQ, An RH. Externalization of phosphatidylserine via multidrug resistance 1 (MDR1)/P-glycoprotein in oxalate-treated renal epithelial cells: implications for calcium oxalate urolithiasis. Int Urol Nephrol 2016;48:175-81.
https://doi.org/10.1007/s11255-015-1155-1
Meimaridou E, Jacobson J, Seddon AM, NoronhaDutra AA, Robertson WG, Hothersall JS. Crystal and microparticle effects on MDCK cell superoxide production: oxalate-specific mitochondrial membrane potential changes. Free Radic Biol Med 2005;38:1553-64.
https://doi.org/10.1016/j.freeradbiomed.2005.02.020
Khan SR. Crystal-induced inflammation of the kidneys: results from human studies, animal models, and tissue-culture studies. Clin Exp Nephrol 2004;8:75-88. https://doi.org/10.1007/s10157-004-0292-0
Cao LC, Honeyman TW, Cooney R, Kennington L, Scheid CR, Jonassen JA. Mitochondrial dysfunction is a primary event in renal cell oxalate toxicity. Kidney Int 2004;66:1890-900.
https://doi.org/10.1111/j.1523-1755.2004.00963.x
Sun XY, Ouyang JM, Li YB, Wen XL. Mechanism of cytotoxicity of micron/nano calcium oxalate monohydrate and dihydrate crystals on renal epithelial cells. RSC Adv 2015;5: 45393-406.
https://doi.org/10.1039/C5RA02313K
Sun XY, Ouyang JM, Yu K. Shape-dependent cellular toxicity on renal epithelial cells and stone risk of calcium oxalate dihydrate crystals. Sci Rep 2017;7:7250. https://doi.org/10.1038/s41598-017-07598-7
Vanachayangkul P, Byer K, Khan S, Butterweck V. An aqueous extract of Ammi visnaga fruits and its constituents khellin and visnagin prevent cell damage caused by oxalate in renal epithelial cells. Phytomedicine 2010;17: 653-8. https://doi.org/10.1016/j.phymed.2009.10.011
Kanlaya R, Khamchun S, Kapincharanon C, Thongboonkerd V. Protective effect of epigallocatechin-3-gallate (EGCG) via Nrf2 pathway against oxalate-induced epithelial mesenchymal transition (EMT) of renal tubular cells. Sci Rep 2016;6:30233. https://doi.org/10.1038/srep30233
Sun XY, Ouyang JM, Li YB, Wen XL. Mechanism of cytotoxicity of micron/nano calcium oxalate monohydrate and dihydrate crystals on renal epithelial cells. RSC Adv 2015;5:45393-406.
https://doi.org/10.1039/C5RA02313K
Kizivat T, Smolic M, Maric I, Tolusic LM, Smolic R, Bilic C, I, et al. Antioxidant pre-treatment reduces the toxic effects of oxalate on renal epithelial cells in a cell culture model of urolithiasis. Int J Environ Res Public Health 2017;14.
Khan A, Bashir S, Khan SR, Gilani AH. Antiurolithic activity of Origanum vulgare is mediated through multiple pathways. BMC Complement Altern Med 2011;11:96. https://doi.org/10.1186/1472-6882-11-96
Goufo P, Trindade H. Rice antioxidants: phenolic acids, flavonoids, anthocyanins, proanthocyanidins, tocopherols, tocotrienols, gammaoryzanol, and phytic acid. Food Sci Nutr 2014;2:75-104.
https://doi.org/10.1002/fsn3.86
Xu Z, Hua N, Godber JS. Antioxidant activity of tocopherols, tocotrienols, and gammaoryzanol components from rice bran against cholesterol oxidation accelerated by 2,2'-azobis(2-methylpropionamidine) dihydrochloride. J Agric Food Chem 2001; 49:2077-81.
https://doi.org/10.1021/jf0012852
Jang HH, Park MY, Kim HW, Lee YM, Hwang KA, Park JH, et al. Black rice (Oryza sativa L.) extract attenuates hepatic steatosis in C57BL/6 J mice fed a high-fat diet via fatty acid oxidation. Nutr Metab (Lond) 2012;9:27. https://doi.org/10.1186/1743-7075-9-27
Sangkitikomol W, Tencomnao T, Rocejanasaroj A. Effects of Thai black sticky rice extract on oxidative stress and lipid metabolism gene expression in HepG2 cells. Genet Mol Res 2010;9:2086-95.
https://doi.org/10.4238/vol9-4gmr912
Sangkitikomol W, Tencomnao T, Rocejanasaroj A. Antioxidant effects of anthocyanins-rich extract from black sticky rice on human erythrocytes and mononuclear leukocytes. Afr J Biotechnol 2010;9:8222-9. https://doi.org/10.5897/AJB10.827
Sutharut J, Sudarat J. Total anthocyanin content and antioxidant activity of germinated colored rice. Int Food Res J 2012;19:215-21.
Sah SK, Kushwaha UKS.Book review: Black rice: Research, history, and development. Adv Plants Agric Res 2016;5:00165 https://doi.org/10.15406/apar.2016.05.00165
Markakis P. Anthocyanins as food colors. New York: Academic Press; 1982.
https://doi.org/10.1016/B978-0-12-472550-8.50013-5
Tian S, Nakamura K, Kayahara H. Analysis of phenolic compounds in white rice, brown rice, and germinated brown rice. J Agric Food Chem 2004;52:4808-13. https://doi.org/10.1021/jf049446f
Nam SH, Choi SP, Kang MY, Kozukue N, Friedman M. Antioxidative, antimutagenic, and anticarcinogenic activities of rice bran extracts in chemical and cell assays. J Agric Food Chem 2005;53:816-22. https://doi.org/10.1021/jf0490293
Laokuldilok T, Shoemaker CF, Jongkaewwattana S, Tulyathan V. Antioxidants and antioxidant activity of several pigmented rice brans. J Agric Food Chem 2011;59:193-9.
https://doi.org/10.1021/jf103649q
Hu C, Zawistowski J, Ling W, Kitts DD. Black rice (Oryza sativa L. indica) pigmented fraction suppresses both reactive oxygen species and nitric oxide in chemical and biological model systems. J Agric Food Chem 2003;51: 5271-7. https://doi.org/10.1021/jf034466n
Htwe NN, Srilaong V, Tanprasert K, Tongchitpakdee S, Kanlayanarat S, Uthairatanakij A. Effects of storage time and temperature on radical scavenging activities and bioactive compounds in colored rice varieties. J Food Agr Environ 2010;8:26-31.
Fargue S, Harambat J, Gagnadoux MF, Tsimaratos M, Janssen F, Llanas B, et al. Effect of conservative treatment on the renal outcome of children with primary hyperoxaluria type 1. Kidney Int 2009;76:767-73. https://doi.org/10.1038/ki.2009.237
Moe OW, Xu LHR. Hyperuricosuric calcium urolithiasis. J Nephrol 2018;31:189-96.
https://doi.org/10.1007/s40620-018-0469-3
Tosukhowong P, Yachantha C, Sasivongsbhakdi T, Ratchanon S, Chaisawasdi S, Boonla C, et al. Citraturic, alkalinizing and antioxidative effects of limeade-based regimen in nephrolithiasis patients. Urol Res 2008;36:149-55. https://doi.org/10.1007/s00240-008-0141-9
Tosukhowong P, Kulpradit P, Chaiyarit S, Ungjareonwattana W, Kalpongnukul N, Ratchanon S, et al. Lime powder treatment reduces urinary excretion of total protein and transferrin but increases uromodulin excretion in patients with urolithiasis. Urolithiasis 2017 Jun 5. doi: 10.1007/s00240-017-0986-x. https://doi.org/10.1007/s00240-017-0986-x
Ruiz-Agudo E, Burgos-Cara A, Ruiz-Agudo C, Ibanez-Velasco A, Colfen H, RodriguezNavarro C. A non-classical view on calcium oxalate precipitation and the role of citrate. Nat Commun 2017;8:768.
https://doi.org/10.1038/s41467-017-00756-5
Chen XQ, Nagao N, Itani T, Irifune K. Anti-oxidative analysis, and identification and quantification of anthocyanin pigments in different coloured rice. Food Chem 2012;135:2783-8.
https://doi.org/10.1016/j.foodchem.2012.06.098
Welch CR, Wu Q, Simon JE. Recent advances in anthocyanin analysis and characterization. Curr Anal Chem 2008;4:75-101. https://doi.org/10.2174/157341108784587795
Hall JA, Brockman JA, Davidson SJ, MacLeay JM, Jewell DE. Increased dietary long-chain polyunsaturated fatty acids alter serum fatty acid concentrations and lower risk of urine stone formation in cats. PLoS One 2017;12: e0187133. https://doi.org/10.1371/journal.pone.0187133
Yasir F, Wahab AT, Choudhary MI. Protective effect of dietary polyphenol caffeic acid on ethylene glycol-induced kidney stones in rats. Urolithiasis 2018;46:157-66.
https://doi.org/10.1007/s00240-017-0982-1
Seeger H, Kaelin A, Ferraro PM, Weber D, Jaeger P, Ambuehl P, et al. Changes in urinary risk profile after short-term low sodium and low calcium diet in recurrent Swiss kidney stone formers. BMC Nephrol 2017;18:349. https://doi.org/10.1186/s12882-017-0755-7
Li Y, Pan J, Zhang Y, Chang Y, Yang X, Yang B, et al. Effects of small molecules water that may retard kidney stone formation. Int Urol Nephrol 2018;50:225-30. https://doi.org/10.1007/s11255-017-1769-6
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