Antioxidant activity of Caesalpinia sappan heartwood extracts against oxidative stress induced by hydrogen peroxide in MRC-5 cell lines

Aylada  Kaenu; Supranee  Kongkham

Authors

Aylada Kaenu Faculty of Medicine, Thammasat University, Pathumthani Thailand
Supranee Kongkham Faculty of Medicine, Thammasat University, Pathumthani Thailand and Faculty of Allied Health Sciences, Nakhonratchasima College, Nakhonrachasima, Thailand

Keywords:

Antioxidant activity, Caesalpinia sappan, human lung fibroblast MRC-5 cells, hydrogen peroxide

Abstract

Background: Caesalpinia sappan (C. sappan) heartwood extract is a potentially effective antioxidant against oxidative stress. A few studies have been published on cell-based assay, especially in normal human lung fibroblast cells.

Objectives: To investigate how the concentration of ethanol used for extraction influence the antioxidant capacity of C. sappan heartwood extracts of against oxidative stress in human lung fibroblast MRC-5.

Methods: The antioxidant activity of crude extracts of C. sappan heartwood extracts were analyzed by chemi-based assayand cell-based assay (in-vitro lipid peroxidation), catalase (CAT), and superoxide dismutase (SOD) in MRC-5 cells.

Results: The results showed that the antioxidant effect of C. sappan heartwood extracts significantly depended on the percentage concentration of ethanol used for extraction. The crude extract prepared with 75% ethanol had the highest antioxidant effect. This extract had a total phenolic content of 741.8 mg GAE/ g CS extract, and it could reduce activities of CAT and SOD in H2O2-treated MRC-5 cells. The C. sappan extract could also inhibit lipid peroxidation in MRC-5 cells treated with H2O2.

Conclusion: These findings indicate that C. sappan heartwood extracts have a good antioxidative potential to reduce oxidative stress and could be used as a dietary supplement for maintaining health.

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