Modified QuEChERS in blood sample preparation for drug abuse determination by LC-MS/MS

Authors

  • Bundhita Boonchaleaw Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Nat Tansrisawad Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
  • Udomsak Hoonwijit Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

Keywords:

Modified QuEChERS, drug abuse, LC-MS/MS

Abstract

Background: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) becomes a common technique in the routine forensic toxicological analysis because of its accurate result. Solid-phase extraction (SPE) and liquid-liquid extraction (LLE) are the sample preparation methods having disadvantages from time-consuming and using a high quantity of harmful substances. Therefore, modified QuEChERS has been developed for routine toxicological laboratory for simpler sample preparation that could save time and decrease the use of toxic solvent for routine laboratory.

Objective: To validate the modified QuEChERS technique for the blood sample preparation and to determine drug abuse in the blood sample using LC-MS/MS.

Methods: Amphetamine, methadone, midazolam, morphine and triazolam were added to the blood. Modified QuEChERS was used for blood sample preparation and the analysis by LC-MS/MS was performed. Validation of the technique was tested and the application to the routine cases was done.

Results: The chromatographic data of the interested abused drugs were obtained within 14 minutes. Modified QuEChERS extracted products could recover the 5 drugs in which the coefficient of determination of the calibration curves was higher than 0.99 and no influence of other drugs was found in the selectivity test.

Conclusion: Modified QuECHERS is suitable for sample preparation before analysis with LC-MS/MS in routine forensic toxicology laboratory.

Downloads

Download data is not yet available.

Downloads

Published

2023-07-19

How to Cite

1.
Boonchaleaw B, Tansrisawad N, Hoonwijit U. Modified QuEChERS in blood sample preparation for drug abuse determination by LC-MS/MS. Chula Med J [Internet]. 2023 Jul. 19 [cited 2024 Oct. 12];65(1). Available from: https://he05.tci-thaijo.org/index.php/CMJ/article/view/117

Similar Articles

You may also start an advanced similarity search for this article.